PfSHMTc immunofluorescence images showing localization in the mitochondrion. (A) Mid-trophozoite showing the association of a small mitochondrion with PfSHMTc fluorescence. (B) Early schizont showing association of an enlarged globular mitochondrion with a region of more intense PfSHMTc fluorescence. (C) Late schizont showing very little co-localization of mitochondria with areas of PfSHMTc fluorescence. Mitochondria are closely aligned to nuclei and show some co-localization with YOYO1 staining (scale bars 3 μm). The associated table shows the percentage volume (V%) and material (M%) co-localization data for PfSHMTc (Sc) and MitoTracker (MIT) fluorescence.