PfSHMTc immunofluorescence images showing localization in the apicoplast. (A) Mid-trophozoite showing the co-localization of plastid specific fluorescence with PfSHMTc fluorescence. (B) Early mitotic schizont showing very marked co-localization of plastid specific fluorescence (enlarged globular apicoplast) with PfSHMTc fluorescence. (C) Mitotic schizont showing very marked co-localization of plastid specific fluorescence with PfSHMTc fluorescence. The plastid here is in the early stages of elongation. Note also the small punctate concentration of PfSHMTc fluorescence on the periphery of the unstained region of the parasite corresponding to the pigment vacuole. (D) Mitotic schizont developmentally a little later than (C) showing a mitochondrion in the early stages of ramification. The area of intense PfSHMTc fluorescence follows the 'Y' shape of the mitochondrion closely (scale bars 3 μm, except (D) which is 2 μm). The associated table shows the percentage volume (V%) and material (M%) co-localization data for PfSHMTc (Sc) and acyl carrier protein (ACP) fluorescence.