Figure 6
Effect of adenine on hypoxanthine uptake into uninfected and P. falciparum -infected human erythrocytes. Uptake of 0.5 μM [3H]-hypoxanthine, expressed as pmol(107 cells)-1, was determined in (A) uninfected and (B) P. falciparum-infected RBC at room temperature. Solid squares, no inhibitor control; open circles, 1 mM unlabelled hypoxanthine added; solid down triangles, 1 mM adenine added. The rate of uninhibited hypoxanthine uptake in infected and uninfected RBC was 0.0086 ± 0.0006 and 0.0045 ± 0.0001 pmol(107 cells)-1s-1, respectively; rates in the presence of 1 mM adenine and hypoxanthine were not significantly different from zero (P > 0.05; F-test). Experiments were performed in triplicate; error bars represent SEM.