Intradermal immunization of mice with radiation-attenuated sporozoites of Plasmodium yoelii induces effective protective immunity

Table 1 Effect of immunization protocols with Plasmodium yoelii attenuated sporozoites on development of protective immunity

Route of Immunization	Exp't 1	Exp't 2	Exp't 3	Total
Non-Immunized Controls	6/6 (0%)^a [PP = 3.0]^b	6/6 (0%) [PP = 3.3]	6/6 (0%) [PP = 3.3]	18/18 (0%) [PP = 3.3]
IV^c-Immunized	0/6 (100%)	0/6 (100%)	1/6 (83%) [PP = 6.0]	1/18 (94%) [PP = 6.0]
ID^d-Immunized	3/6 (50%) [PP = 5.0]	1/6 (83%) [PP = 9.0]	0/6 (100%)	4/18 (78%) [PP = 6.0]
ID-Immunized + Imiquimod	1/6 (83%) [PP = 5.0]	1/6 (83%) [PP = 4.0]	4/6 (33%) [PP = 4.2]	6/18 (67%) [PP = 4.3]
ID-Immunized + Tape Stripping	1/6 (83%) [PP = 4 0]	0/6 (100%)	0/6 (100%)	1/18 (94%) [PP = 4.0]

^a Results of challenge by mosquito injection of sporozoites. # of mice with blood infection/# challenged; (% protected in parentheses)
^b [Mean prepatent period in days for positive mice]
^c IV = intravenous
^d ID = intradermal

ISSN: 1475-2875