Schizont-irbc fraction of isolates in eleven independent experiments. Total numbers of 2.5 × 108 to 3 × 109 rbc from cultures with 5-15% total parasitemia were applied to the HGMS column. Increasing the total number or schizont-irbc applied to the column resulted in higher fractions of schizont-irbc in total irbc. Total irbc purities ranged from 93.20 to 98.40% (Mean 95.69%, S.D. 1.52).