Optimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cells

Malaria Journal

Table 1 Yield and purity of isolates in six independent experiments

Experiment no.	Yield [total no. of irbc purified]	Purity irbc/total cells [%]
1	12,787,500	98.46
2	16,937,500	94.23
3	11,287,500	95.42
4	9,962,500	94.87
5	11,462,500	96.43
6	10,325,000	95.05
Mean	12,127,083	95.74
S.D.	2,600,000	1.38

5 × 10⁸ of rbc from cultures with 5-10% parasitemia were applied to the HGMS column. Experiments were performed from different cultures on different days. Purity was determined by flow cytometry, as described in material and methods.

ISSN: 1475-2875