Impact of the charge of pigment in the placenta. For PfHRP2+ placenta, the pigment deposit was plotted as: "no pigment" (No); "low load of pigment" (low) for less than 3 pigments for 10 field (×400); "high load of pigment" (high) for more than 3 pigments per field; "control group" (Ctrl) for PfHRP2- placenta. A) Relation between charge in pigment and birth weight of the newborn (g), B) Relation between charge in pigment and haemoglobin level of the mother (g/100 ml), C) Relation between charge in pigment and count of CD68+ and HLA-DR cells in the placenta (number of cells/100 fields at magnification ×400). D) Relation between charge in pigment and expression level of the four genes (RQ = Relative Quantity = 2-(deltadelta Ct)). NS = not significant, (*) means significant when compared with the control group.