Figure 4

P. yoelii -infected erythrocytes inhibit DC maturation in response to the TLR4 ligand LPS. Bone-marrow derived DC were incubated for 24 h in different conditions and then further stimulated with LPS (black bars) or not (white bars) for 18 h. CD86 and CD40 expression determined by flow cytometry are expressed as fold-to-control (DC alone). CD40 (A) and CD86 (B) expression after incubation of DC for 24 h with RBC or P. yoelii-iRBC at different ratios, as well as RBC or iRBC lysates (lys) and with or without LPS for another 18 h. Data correspond to the average of triplicate samples ± standard deviation and figures are representative of one out of two independent experiments.