Transmission blocking activity of a standardized neem (Azadirachta indica) seed extract on the rodent malaria parasite Plasmodium berghei in its vector Anopheles stephensi

Table 3 P. berghei oocyst number/mosquito and development stage proportions after a secondary, treated blood meal.

*Treatment*	*Replication*	Stage of development*			*Total (CI95%)*	*Examined mosquitoes*
*Treatment*	*Replication*	*s1 (%)*	*s2 (%)*	*s3 (%)*	*Total (CI95%)*	*Examined mosquitoes*
control	1	25 (38)	23 (37)	12 (25)	70 (50 - 98)	27
	2	15 (33)	12 (31)	13 (36)	54 (42 - 70)	92
NA 50 mg/kg	1	10 (36)	10 (36)	8 (28)	31 (19 - 49)	23
	2	30 (42)	21 (33)	11 (25)	82 (68 - 100)	112
AZA 50 mg/kg	1	19 (34)	20 (35)	18 (31)	61 (40 - 93)	27

Secondary blood meals were carried out on mice treated with the indicated products and doses on day 7 after mosquito infection. Oocysts were evaluated on day 3 after the secondary blood meal (day 10 post infection). *s1: immature oocysts, before the formation of sporoblasts (fig. 1A); s2: immature oocysts, with visible sporoblasts and budding sporozoites (fig. 1 B1 - B2); s3: mature oocysts containing fully developed sporozoites (fig. 1C). Geometric means, only positive mosquitoes included.

ISSN: 1475-2875