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  • Oral presentation
  • Open Access

A micro-bead device to explore Plasmodium falciparum-infected, spherocytic or aged red blood cells prone to mechanical retention by spleen endothelial slits

  • 1, 2, 3,
  • 1, 2,
  • 3,
  • 4,
  • 5,
  • 1, 2,
  • 3, 6,
  • 1, 2,
  • 7,
  • 8,
  • 8,
  • 8,
  • 9,
  • 10,
  • 3, 6,
  • 3, 6,
  • 11,
  • 12,
  • 1, 2,
  • 1, 2 and
  • 3, 6
Contributed equally
Malaria Journal20109 (Suppl 2) :O10

https://doi.org/10.1186/1475-2875-9-S2-O10

  • Published:

Keywords

  • Blood Cell
  • Infectious Disease
  • Plasmodium
  • Medical Application
  • Retention Rate

Experimental tools to identify human red blood cells (RBC) prone to mechanical retention upstream from the spleen venous sinus inter-endothelial slits are currently suboptimal. We designed a micro-bead device mimicking the geometry of the human narrow and short inter-endothelial slits. Upon filtration through a mixture of 5-25 μm diameter micro-beads, Plasmodium falciparum-hosting RBC (Pf-RBC) were retained in a parasite developmental stage-dependent way, the retention rates of a subset of ring-RBC being similar in micro-beads and in isolated-perfused human spleens. We found that this retention might be linked principally to the reduced surface-area-to-volume ratio of Pf-RBC. Interestingly, other rigid RBC, such as heat-treated RBC, and RBC from hereditary spherocytosis patients were also retained in micro-beads without any hemolysis. Micro-beads allow (i) depletion of heterogeneous RBC population from its rigid-RBC subpopulation ii) characteriziation of distinct molecular signatures of rigid versus deformable RBC subpopulations. This simple method portends wide medical applications, such as improving the quality of stored RBC concentrates prior to transfusion.

Notes

Authors’ Affiliations

(1)
Département de Parasitologie Mycologie, Institut Pasteur, Unité d'Immunologie Moléculaire des Parasites, F- 75015 Paris, France
(2)
CNRS, URA2581 Paris, France
(3)
INSERM - UPMC (Paris 6 University) UMRs945, F-75013 Paris, France
(4)
Fond Ackermann, Fondation de France
(5)
Department of Pediatrics, Necker Hospital, AP-HP, F-75015 Paris, France
(6)
Department of Parasitology, Pitié Salpétrière Hospital, AP-HP, F-75013 Paris, France
(7)
Department of Haematology, Kremlin-Bicêtre Hospital, AP-HP, F-94270 Le Kremlin-Bicêtre, France
(8)
Department of Surgery, Beaujon Hospital, AP-HP, F-92110 Clichy, France
(9)
Department of Pathology, Beaujon Hospital, AP-HP, F-92110 Clichy, France
(10)
Department of Surgery, Saint-Antoine Hospital, AP-HP, F-75018 Paris, France
(11)
Département de Parasitologie Mycologie, Institut Pasteur, mmunophysiologie et Parasitisme Intracellulaire, F-75015 Paris, France
(12)
New York Blood Centre, New York, NY 10065, USA

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