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Characterization and comparative sequence analyzes of GABA receptor gene in Asian main malaria mosquito, Anopheles stephensi
Malaria Journal volume 9, Article number: O15 (2010)
The mutations occurring in receptor genes of Gama Amino Butyric Acid (GABA) play important roles in resistance to cychlodien insecticides in mosquitoes. Here we report the sequence analysis of the Rdl gene from Asian main malaria mosquito, Anopheles stephensi, using specific primers in the polymerase chain reaction.
Method and results
Mosquitoes were collected from Fars, Hormozgan, Kerman and Sistan & Baluchistan provinces of Iran. A 256bp sequence of GABA receptor showed 99-100% similarity between An. stephensi populations in Iran. Gas chromatography count was 41.63% with 86 amino acid sequences. Alteration in amino acid sequence that has been found in An. stephensi populations will be discussed in detail, in comparison with An. gambiae.
Understanding of molecular structure of resistance can provide baseline data to evaluate population genetics of pesticide resistance, which will be applicable in operational vector control measures and sterile insect technique.
Thompson M, Shotkoski F, ffrench-Constant R: Cloning and sequencing of the cyclodiene insecticide resistance gene from the yellow fever mosquito Aedes aegypti. Conservation of the gene and resistance associated mutation with Drosophila. FEBS Lett. 1993, 325: 187-90. 10.1016/0014-5793(93)81070-G.
Buckingham SD, Biggin PC, Sattelle BM, Brown LA, Sattelle DB: Insect GABA receptors: splicing, editing, and targeting by antiparasitics and insecticides. Mol Pharmacol. 2005, 68: 942-51. 10.1124/mol.105.015313.
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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Gholizadeh, S., Karimi, A., Bayat, N. et al. Characterization and comparative sequence analyzes of GABA receptor gene in Asian main malaria mosquito, Anopheles stephensi. Malar J 9 (Suppl 2), O15 (2010). https://doi.org/10.1186/1475-2875-9-S2-O15
- Polymerase Chain Reaction
- Amino Acid Sequence
- Control Measure
- Vector Control