Volume 9 Supplement 2

Parasite to Prevention: Advances in the understanding of malaria

Open Access

A comprehensive survey of protein palmitoylation in late blood-stage Plasmodium falciparum

  • Matthew L Jones1,
  • Mark O Collins1,
  • Jyoti Choudhary1 and
  • Julian C Rayner1
Malaria Journal20109(Suppl 2):O20

https://doi.org/10.1186/1475-2875-9-S2-O20

Published: 20 October 2010

Protein palmitoylation, the addition of a 16-Carbon saturated fatty acid to cysteine residues, is a common post-translation modification of both peripheral and integral membrane proteins. As a reversible modification, palmitoylation is essential for the dynamic regulation of protein function -controlling, in many cases, protein localisation both to and within specific membrane domains, protein stability, and protein activity.

Using two complementary techniques, one relying on the exchange of palmitoyl groups with a biotin linker and the other relying on metabolic labelling of cells with a palmitic acid analogue, we have purified the total catalogue of palmitoylated protein from Plasmodium falciparum schizonts. The use of these two complementary techniques in tandem with SILAC (stable isotope labeling by amino acids in cell culture) based mass spectrometry (MS) to allow robust quantitative MS have allowed the identification of more than 250 palmitoylated proteins in late blood-stage P. falciparum parasites. This represents the first global analysis of protein palmitoylation in any Plasmodium parasite and has identified palmitoyl-proteins involved in protein secretion, protein export, drug resistance, signalling, development, and invasion. Furthermore, we have found that palmitoylation is essential for the completion of the blood-stage life cycle and that this modification directly regulates the stability of at least two proteins essential for invasion, PfGAP45 and PfMTIP.

Authors’ Affiliations

(1)
Sanger Institute Malaria Programme, Wellcome Trust Sanger Institute

Copyright

© Rayner et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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