Skip to content


Malaria Journal

Open Access

Antimalarial activity of ulein and proof of its action on the Plasmodium falciparum digestive vacuole

  • Alaíde Braga de Oliveira1,
  • Maria Fâni Dolabela2,
  • Marinete Marins Póvoa3,
  • Cid Aimbiré M Santos4 and
  • Fernando de Pilla Varotti5
Malaria Journal20109(Suppl 2):O9

Published: 20 October 2010


MalariaAlkaloidQuinolinePlasmodium FalciparumChloroquine


For thousands of years, plants have formed the basis of traditional medicine systems. The first drug to be used against malaria was quinine, which was isolated from South American Cinchona spp., in 1820. More recently, artemisinin, isolated in China from Artemisia annua L. and derivatives have been used successfully against malaria that has become resistant to chloroquine. We have been investigating plants traditionally used in Brazil to treat malaria and fevers.

Materials and methods

Screening of plant extracts for inhibition of Plasmodium falciparum growth was assayed by the [3H]-hypoxanthine methodology. EtOH extract from Aspidosperma parvifolium was shown to be active (W2: IC50 = 42.51 ± 6.33 μg/ml) and has afforded the alkaloid ulein, which has shown a good antiplasmodial activity (W2: IC50 = 0.98 ± 0.20 μg/ml). Possible targets for ulein have been investigated by confocal microscopy using a proton fluorescent probe (BCECF-AM) in P. falciparum synchronous culture of W2-infected red blood cells by comparison with mefloquine (MQ) and bafilomycin A1 (BAF). Dynamic imaging was performed with the LSM 510 laser-scanning microscope (Carl Zeiss), using LSM 510 software (version 2.5), in the Axiovert 100 M microscope equipped with a 63xoil immersion objective. Software-based analysis of data allowed fluorescence imaging in a selected cell as a function of time.


Dynamic images have shown that ulein (5 ng/ml), was able to mobilize protons altering the pH gradient in the digestive vacuole (DV), like MQ, a weak-base antimalarial quinoline. However, after the addition of ulein, BAF (4 μM), an inhibitor of the H+ pump from acidic compartments of eukaryotic cells, had no action on the DV suggesting that it is a target for ulein action.


This work shows that ulein is able to act on the DV, probably due to its alkaloid structure. Whether there is a participation of PfCRT, PfMDR1, and PfATPase6 in ulein action is still an open question. Its action on calcium homeostasis needs to be further investigated. These data disclose ulein as a potential antimalarial drug.



Funding was provided by FAPEMIG, CNPq, MS, Brazil.

Authors’ Affiliations

Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
Universidade Federal do Pará, Belém, Brazil
Instituto Evandro Chagas, Belém, Brazil
Universidade Federa do Paraná, Curitiba, Brazil
Universidade Federal de São João Del Rei, Divinópolis, Brazil


© de Oliveira et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.