Volume 9 Supplement 2

Parasite to Prevention: Advances in the understanding of malaria

Open Access

Anti-peptide antibodies in the diagnosis of malaria

  • J P Dean Goldring1,
  • Ramona Hurdayal1 and
  • David Choveaux1
Malaria Journal20109(Suppl 2):P12

https://doi.org/10.1186/1475-2875-9-S2-P12

Published: 20 October 2010

Lactate dehydrogenase is one of the antigens targeted in lateral flow immunochromatographic rapid diagnostic tests for the diagnosis of malaria. Unique diagnostic peptide targets based on the respective amino-acid sequences to differentiate between P. falciparum and P. vivax lactate dehydrogenase were chosen, synthesized and coupled to rabbit albumin carrier. Anti-peptide antibodies were raised in chickens against the peptides and against each of the recombinant proteins and affinity purified. An antibody against a common epitope detected recombinant P. falciparum, P. vivax and P. yoelii and native P. falciparum and P. vivax protein lactate dehydrogenase. Antibodies against species specific lactate dehydrogenase epitopes differentiated between P. falciparum and P. vivax lactate dehydrogenase. The study supports an anti-peptide antibody approach for the design of malaria diagnostic reagents.

Authors’ Affiliations

(1)
Ikechukwu Achilonu Biochemistry, University of KwaZulu-Natal

Copyright

© Dean Goldring et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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