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Table 2 The means of CI 50 of the SYBR green based in vitro anti-malarial drugs assay according to protocols

From: Drying anti-malarial drugs in vitro tests to outsource SYBR green assays

Clone/drugs

Protocol standard

New Protocol (test)

P

Mean CI 50 (nM)

95% CI

Mean CI 50 (nM)

95% CI

3D7

     

CQ

31.10 ± 6.02

24.28-37.91

32.43 ± 4.31

26.22-35.97

0.77

DHA

2.30 ± 0.68

1.53-3.06

1.93 ± 0.43

1.81-2.78

0.47

PYD

8.82 ± 1.17

7.49-10.14

8.89 ± 1.54

7.07-10.56

0.97

PPQ

26.33 ± 1.71

21.38-28.27

25.66 ± 6.82

15.61-34.4

0.88

W2

     

CQ

467.66 ± 51.18

409.74-525.57

500.66 ± 87.22

368.96-566.35

0.60

DHA

1.94 ± 1.03

0.77-3.10

1.81 ± 0.99

0.81-3.06

0.81

PYD

5.35 ± 1.27

3.90-6.79

4.33 ± 1.21

3.98-6.71

0.37

PPQ

19.50 ± 2.91

16.20-22.79

17.20 ± 3.14

15.94-23.05

0.40

HB3

     

CQ

37.92 ± 6.66

30.38-45.47

50.28 ± 16.77

31.30-69.26

0.33

DHA

2.10 ± 0.97

0.99-3.20

1.99 ± 0.41

1.53-2.46

0.87

PYD

11.78 ± 2.36

9.10-14.46

12.51 ± 3.40

8.65-16.36

0.70

PPQ

21.70 ± 1.76

19.70-23.70

18.51 ± 2.24

15.97-21.06

0.12

7G8

     

CQ

416.98 ± 92.57

311.82-521.33

420.55 ± 96.04

311.87-529.24

0.18

DHA

0.65 ± 0.17

0.45-0.85

0.73 ± 0.12

0.58-0.87

0.56

PYD

4.43 ± 1.39

2.84-6.01

5.05 ± 0.34

4.66-5.44

0.52

PPQ

17.82 ± 3.57

13.77-21.87

15.98 ± 1.22

14.59-17.38

0.47

  1. CQ = Chloroquine; DHA = Dihydroartemisinin; PPQ = Piperaquine; PYD = Pyronaridine; SD = Standard deviation, nM = nanomolar.