Inhibitory effect of
on erythroid cell growth and cell cycle. gECs, 5-day old, were cultured with IE/UE lysate at a ratio of 1:10 (gEC:IE/UE) for 24, 48 and 72 h. (a) Cell growth is presented as means ± SD of percentage of control, compared with a control containing medium. (b) Cell death is illustrated as means ± SD of percentage of dead cells, and (c) cell cycle is illustrated as means ± SD of percentage of DNA content in each phase of G0, G0/G1, S, G2/M and > 4n, using propidium iodide staining. Means ± SD were calculated from five independent experiments. *P-value < 0.01 compared with media control.