Schematic diagram and protein expression of PvRALP1-Ecto and PvRALP1-Tr. (A) Schematic diagram of PvRALP1-Ecto and the central domain comprising PvRALP1-Tr. (B) Expression and purification of recombinant PvRALP1-Ecto and (C) PvRALP1-Tr fragments. Arrowheads indicate specific bands for each recombinant protein. M, protein marker; T, total translation mix; S: supernatant; P, pellet; Ft: flow through; E1, elution fraction 1; E2, elution fraction 2; E3, elution fraction 3.