Western blot analysis of recombinant PvRALP1-Ecto, PvRALP1-Tr, and schizont lysate probed with antisera to PvRALP1-Ecto and PvRALP1-Tr, and sera from P. vivax malaria patients and healthy controls. (A) PvRALP1-Ecto and PvRALP1-Tr were probed respectively with anti-GST antibody (Gst), immune mouse sera (M), pooled P. vivax patient sera (P), anti-His antibody (His), immune rabbit sera (R), nonimmune mouse sera (N), and sera from noninfected humans (H). (B) Recognition of PvRALP1 antigen in the parasite lysate with mouse (1) and rabbit (2) antisera against PvRALP1-Tr, preimmune mouse sera (3), and preimmune rabbit serum (4), respectively. Arrowheads indicate the target bands for native and recombinant proteins and putative processed fragments. The asterisk indicates a nonspecific band present in each lane.