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Figure 4 | Malaria Journal

Figure 4

From: Development of two novel high-throughput assays to quantify ubiquitylated proteins in cell lysates: application to screening of new anti-malarials

Figure 4

Optimization of the components of the TUBE-DELFIA assay. X axis of panels A and C are represented in logarithmic scale. A. Titration of TUBEs with saturating concentration of proteins from isolated P. falciparum cell extracts (10 μg), using anti-ubiquitin FK2 and europium antibodies at 1:200 dilution. B. TUBE-DELFIA assay using a saturating concentration of proteins from isolated P. falciparum cell extracts (10 μg), 30 ng TUBEs and three different concentrations of anti-ubiquitin FK2 and secondary europium antibodies. A control without proteins was used to determine the background signal. C. Haematocrit titration of iRBCs with schizonts at 100% parasitaemia treated with MG132 inhibitor (1.5 μM), using the conditions previously established (30 ng TUBEs, 1:100 FK2 and 1:200 europium antibodies).

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Malaria Journal

ISSN: 1475-2875

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