Table 2 pIC50 and maximum percentage of stimulation of proteasome inhibitors in different assays
Proteasome inhibitor | pIC50 reported whole cell | pIC50 AlphaLISA (n = 4) | Maximum% stimulation AlphaLISA | pIC50 DELFIA (n = 4) | Maximum% stimulation DELFIA |
|---|---|---|---|---|---|
Epoxomicin | 8.17 | 7.64 ± 0.09 | 178 ± 47 | 7.74 ± 0.24 | 177 ± 15 |
Bortezomib | 7.05 | 7.95 ± 0.08 | 178 ± 24 | 8.16 ± 0.05 | 180 ± 14 |
Gliotoxin | 5.66 | 5.88 ± 0.73 | 217 ± 53 | 6.12 ± 0.27 | 180 ± 26 |
MG132 | 7.65 | 7.17 ± 0.04 | 236 ± 36 | 7.41 ± 0.21 | 189 ± 55 |
MG115 | 7.01 | 6.88 ± 0.09 | 208 ± 22 | 6.92 ± 0.21 | 185 ± 17 |
Lactacystin | 5.83 | 5.81 ± 0.07 | 217 ± 27 | 6.06 ± 0.22 | 193 ± 32 |
Clasto-lactacystin β lactone | 5.83 | 6.30 ± 0.09 | 195 ± 19 | 6.48 ± 0.18 | 198 ± 17 |