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Table 2 pIC50 and maximum percentage of stimulation of proteasome inhibitors in different assays

From: Development of two novel high-throughput assays to quantify ubiquitylated proteins in cell lysates: application to screening of new anti-malarials

Proteasome inhibitor pIC50 reported whole cell pIC50 AlphaLISA (n = 4) Maximum% stimulation AlphaLISA pIC50 DELFIA (n = 4) Maximum% stimulation DELFIA
Epoxomicin 8.17 7.64 ± 0.09 178 ± 47 7.74 ± 0.24 177 ± 15
Bortezomib 7.05 7.95 ± 0.08 178 ± 24 8.16 ± 0.05 180 ± 14
Gliotoxin 5.66 5.88 ± 0.73 217 ± 53 6.12 ± 0.27 180 ± 26
MG132 7.65 7.17 ± 0.04 236 ± 36 7.41 ± 0.21 189 ± 55
MG115 7.01 6.88 ± 0.09 208 ± 22 6.92 ± 0.21 185 ± 17
Lactacystin 5.83 5.81 ± 0.07 217 ± 27 6.06 ± 0.22 193 ± 32
Clasto-lactacystin β lactone 5.83 6.30 ± 0.09 195 ± 19 6.48 ± 0.18 198 ± 17