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Table 2 Performance indicators of various gametocytocidal assay platforms

From: Nowhere to hide: interrogating different metabolic parameters of Plasmodium falciparum gametocytes in a transmission blocking drug discovery pipeline towards malaria elimination

Assay performance parameters (avg) ATP pLDH PrestoBlue® Luciferase
(n = 4) (n = 4) (n = 4) (n = 4)
S/N >15 000 >300 >900 16 000 (early)
500 (late)
S/B 500 3.2 15 175
Z’-factor (avg) 0.79 0.87 0.91 0.81
%CV (intra-assay) 8.8 % 2.4 % 3.15 % 0.73 %
Avg DHA activity at 1 μM (% inhibition) 37.19 ± 7.67 % 62 ± 6 % 83.48 ± 8.58 % 73.56 ± 5.58 % (avg early/late)
IC50 DHA 14.9 μMa 20 nM 11 nM 43 nM (early)
11 nM (late)
IC50 MB 900 nMa 800 nM - 195 nM (early)
143 nM (late)
  1. DHA Dihydroartemisinin, MB Methylene blue
  2. aunpaired experiments at 24 h
  3. Each assay was performed after 48 h drug exposure and comparative quality control parameters determined, utilising standardization of DHA activity as common factor between all the assay platforms. Methylene blue interferes with the PrestoBlue® assay and was not used as control. Data are from 4 independent experiments (performed in triplicate) for each assay platform