Figure 2

RFLP gel pictures of three markers. a Alu I digestion of Pvcsp PCR products for VK210 type, digested (lane 2, 4, 6, 9–13), undigested (lane 3), marker [lane 7, 8 (100 bp)]. b Bst NI digestion for VK247 type, digested (lane 3–7, 11, 13–17), undigested (lane 1, 2, 8, 10, 12). c Digestion of Pvcsp PCR product with Bbs I for presence of post repeat sequence, digested (lane 2, 4, 6–9, 12), undigested (lane 3, 5, 10, 11, 13), 100 bp marker (lane 1). d Scr FI digestion of Pvcsp product for pre-repeat site, digested (lane 1, 2, 5, 8, 9, 11, 12), undigested (lane 4, 6, 7, 10, 13), 100-bp marker (lane 1). e, f Restriction digestion pattern of Pvmsp-1 F-2 PCR product using Mnl I and Alu I, respectively, 100-bp marker was used in both gel. g, h Digestion pattern of Pvmsp 3α PCR product using Alu I and Hha I, respectively. Presence of a common 1,000-bp fragment observed for Hha I digestion. 50-bp and 100-bp markers (lane 1 of both gel) were used in Alu I and Hha I digestions, respectively.