Figure 3

PfCor-N binds to and bundles F-actin. ai High speed sedimentation of PfCor-N (2 µM) or PfAldolase (2 µM) with 0–10 μM pre-assembled F-actin. Supernatant (S) and pellet (P) fractions shown for representative gel of PfCor-N (Coomassie stained) or PfAldolase (Western, probed with anti-His). ii Densitometry showing % protein co-pelleting with F-actin, values = mean ± SEM (n = 3). bi Supernatant depletion assay showing unspun (U) and spun (S) samples PfCor-N (2 μM) incubated with pre-assembled F-actin (0–20 μM) ii Densitometry of bound PfCor-N based on amount of free actin in solution post-centrifugation, providing a dissociation constant (Kd) = 0.956 μM. c Low-speed sedimentation assay of 2 uM F-actin assembled in presence of 0–10 μM PfCor-N (i), PfAldolase (ii) or α-Actinin with densitometry (iv) shown as % actin remaining in supernatant post-centrifugation, values = mean ± SEM (n = 3).