Membrane association of PfCoroninHA is mediated by PI(4,5)P2. a Western blot of PfCoroninHA. Lanes 1 and 2 supernatant (S) and pellet (P) post hypotonic lysis. Lanes 3 and 4 S and P post Na2CO3 treatment. Top panel probed with anti-HA (Coronin). Second panel probed with anti-PfADF1 . Third panel probed with anti-MSP1p83 (non-membrane associated protein). Fourth panel probed with anti-MSP1-19 (membrane associated GPI anchor). b–d SPR sensograms of (b) PfCor-N (c) PfADF1 and (d) PH-PLCdelta binding to immobilized PI(4,5)P2, shown above panel b. Concentrations of each analyte displayed by curves. Determined Kds = inset in graph.