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Figure 8 | Malaria Journal

Figure 8

From: Plasmodium falciparum coronin organizes arrays of parallel actin filaments potentially guiding directional motility in invasive malaria parasites

Figure 8

Membrane association of PfCoroninHA is mediated by PI(4,5)P2. a Western blot of PfCoroninHA. Lanes 1 and 2 supernatant (S) and pellet (P) post hypotonic lysis. Lanes 3 and 4 S and P post Na2CO3 treatment. Top panel probed with anti-HA (Coronin). Second panel probed with anti-PfADF1 [63]. Third panel probed with anti-MSP1p83 (non-membrane associated protein). Fourth panel probed with anti-MSP1-19 (membrane associated GPI anchor). bd SPR sensograms of (b) PfCor-N (c) PfADF1 and (d) PH-PLCdelta binding to immobilized PI(4,5)P2, shown above panel b. Concentrations of each analyte displayed by curves. Determined Kds = inset in graph.

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