Fig. 9
From: Improvement of culture conditions for long-term in vitro culture of Plasmodium vivax
Purified reticulocytes stained with New Methylene Blue. Reticulocytes from peripheral blood were purified by 19% Nycodenz-KCl. After centrifugation at 3,000×g for 30 min, the reticulocyte-enriched fraction (black arrow, left panel) was collected from the interface and washed three times with RIM. The purified reticulocytes were stained with New Methylene Blue for 15 min before preparing the thin smears. The reticulocytes were identified as the blue reticulin-containing cells (right panel).