Table 1 Summary of limits of detection for HtLAMP-Pg and HtLAMP-Pf for whole blood and filter paper with different DNA extraction protocols
Sample (n = 4) | DNA extraction protocol | Limit of detection nested PCR (parasites/µL) | Limit of detection HtLAMP-Pg (parasites/µL) | Limit of detection HtLAMP-Pf (parasites/µL) |
|---|---|---|---|---|
Whole blood 5 µL | WB-chelex | 5 | 5 | 50 |
WB-chelex-saponin | 25 | 50 | 50 | |
Whole blood 10 µL | WB-chelex | 2.5 | 25 | 50 |
WB-chelex-saponin | 2.5 | 2.5 | 50 | |
Whole blood 20 µL | WB-chelex | 25 | 50 | 50 |
WB-chelex-saponin | 0.5 | 5 | 5 | |
Filter paper 5 µL | Chelex standard protocol (overnight) | 2.5 | 2.5 | 5 |
Chelex rapid protocol (2Â h) | 5 | 25 | 50 |