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Table 1 Summary of limits of detection for HtLAMP-Pg and HtLAMP-Pf for whole blood and filter paper with different DNA extraction protocols

From: A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination

Sample (n = 4) DNA extraction protocol Limit of detection nested PCR (parasites/µL) Limit of detection HtLAMP-Pg (parasites/µL) Limit of detection HtLAMP-Pf (parasites/µL)
Whole blood 5 µL WB-chelex 5 5 50
WB-chelex-saponin 25 50 50
Whole blood 10 µL WB-chelex 2.5 25 50
WB-chelex-saponin 2.5 2.5 50
Whole blood 20 µL WB-chelex 25 50 50
WB-chelex-saponin 0.5 5 5
Filter paper 5 µL Chelex standard protocol (overnight) 2.5 2.5 5
Chelex rapid protocol (2 h) 5 25 50