A simple, high-throughput, colourimetric, field applicable loop-mediated isothermal amplification (HtLAMP) assay for malaria elimination

Table 2 HtLAMP-Pf optical densitometry (OD) results for 77 filter paper samples demonstrating the correlation between the visually detected colour change results for positive samples and their correlation with the positive results based on an OD-derived threshold value

Samples in blue had a blue/cloudy colour change as well as a corresponding positive OD threshold value. Samples in yellow were positive only by OD threshold. Shaded grey wells were negative controls within the plate. OD values for all negative control samples in each plate are used to calculate the OD threshold value (mean plus two standard deviations) which in this example is 0.262

ISSN: 1475-2875