Fig. 2

Time course analysis of PfGEXP5 expression. a Time course of antigen expression after gametocyte induction in a synchronous 3D7 culture. The percentage of mononucleated parasites that are positive for PfGEXP5 alone (green) or for both PfGEXP5-positive and Pfg27 (red) are shown at different time points. These data are typical of experiments performed three times. b Representative immunofluorescence images of (top panel) a ring stage-like parasite at 14 h pi positive for PfGEXP5 (green) and negative for Pfg27 (red), and (lower panel) a pigmented double-positive parasite at 38 h pi. c Immunofluorescence microscopy of ~18-h gametocyte and asexaul ring-infected RBCs labelled with anti-PfGEXP5 (green) and anti-RESA (red) antibodies. Brightfield images (BF) and nuclear staining are shown at right and left, respectively. Scale bar 5 μm