Fig. 6

DC maturation by crude antigens of red blood cell infected with Plasmodium vivax. iDC untreated control DC, Pos RBC malaria positive red blood cell treated DC, Neg RBC normal healthy volunteer’s red blood cell treated DC. DC maturation was assessed by the levels of a PD-L1, b CD86 and c HLA-DR on the DC surface as measured by fluorescence-activated cell sorting (FACS). The relative increase in surface expression is expressed as the mean fluorescence intensity (MFI) of matured DCs over the MFI of iDCs. A representative result of three repeated experiments is shown. Significant differences were determined using the two-way ANOVA (*P < 0.05, **P < 0.01, n = 5)