Non-invasive surveillance for Plasmodium in reservoir macaque species

Malaria Journal

Table 1 Primers and sensitivity for amplifying Plasmodium from faecal samples

ID	Primer sets	Nest?^a	Sequence	Product length (bp)	Gene	Reference for primers
ssrRNA	PfF4595 PfR5019	reamp	GATTACAGCTCCCAAGCAAAC GTTTAGCCAGGAAGTCAGCGTC	424	ssrRNA	This paper
cytb1	PfF3700 PfR4615	nest1a	TGGATGGTGTTTTAGATACATGC GTTTGCTTGGGAGCTGTAATC	915	cytb	This paper
cytb1	PfF3700 PfR4102	nest2a	TGGATGGTGTTTTAGATACATGC GCTGTATCATACCCTAAAG	402	cytb	This paper
cytb2	PfF3368 PfR4102	nest1b	TGCCTAGACGTATTCCTGAT GCTGTATCATACCCTAAAG	734	cytb	This paper
cytb2	PfF3368 PfR3717	nest2b	TGCCTAGACGTATTCCTGAT TATCTAAAACACCATCCACTCCA	349	cytb	This paper
cytb3	qPlasm1f qPlasm1r	nest1c	CTGACTTCCTGGCTAAACTTCC CATGTGATCTAATTACAGAAYAGGA	170	cytb	[32]
cytb3	qPlasm2f qPlasm2r	nest2c	AGAAAACCGTCTATATTCATGTTTG ATAGACCGAACCTTGGACTC	90	cytb	[32]

Primers are all listed in the 5′ to 3′ end
^aNested reactions are indicated: the first primer set is denoted with ‘1’ and the second set is denoted with ‘2’, and nested primer sets can be differentiated by the letter following the number. Sensitivity is only given for the complete nested reaction. The primer set targeting the ssrRNA segment was used twice in a re-amplification reaction to increase yield

ISSN: 1475-2875