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Table 1 Results of non-linear regression analysis of ELISA-measured plasma PfHRP2 concentration vs. digital reading, and of interobserver variability assessment for each of four kits

From: Use of a rapid test to assess plasma Plasmodium falciparum HRP2 and guide management of severe febrile illness

  Core™ Paracheck® First Response® SD Bioline
Digital assessment
 Top (reading units) 1867 1796 807.2 1414
 HillSlope 1.408 1.904 7.058 1.453
 EC50 (ng/ml) 63.14 36.25 75.91 66.99
 EC50 (95 % CI) 48.38–82.40 31.69–41.48 49.63–116.1 47.35–94.80
 Goodness of fit (r2) 0.9082 0.9627 0.5661 0.8516
Visual assessment kappa-statistic
 0—no visible band 0.7931 1 0.7785 0.7857
 1—faint line 0.3197 1 0.3262 0.3056
 2—faint band 0.7869 0.7432 0.6019 0.6296
 3—clear band weaker than control 0.7778 0.9187 0.5879 0.6384
 4—band at least as strong as control 1 0.9664 0.5662 0.85
 Combined 0.8602 0.9377 0.5865 0.7198