Characterization and optimization of the haemozoin-like crystal (HLC) assay to determine Hz inhibiting effects of anti-malarial compounds

Table 1 Growth effect of different ingredients of the HLC assay

Mycoplasma Broth	Tween 80	Lysed blood	Pancreatin (4× USP)	Serum	Growth results
Yes	Yes	Yes	Yes	Yes	Baseline condition
Yes	Yes	Yes (s/h)	Yes	Yes	Like baseline
No	Yes	Yes	Yes	Yes	Little growth, no depolarization
Yes	No	Yes	Yes	Yes	No growth
Yes	Yes	No	Yes	Yes	No growth
Yes	Yes	Yes	No	Yes	No growth
Yes	Yes	Yes	Yes	No	Like baseline
Yes	Yes	Hem	Yes	No	Like baseline
Yes	Yes	Hem	Pan 2.5 %	No	Slightly better than baseline
Yes	Yes	Yes	Pan 2.5 %	No	Slightly better than baseline

Baseline conditions as described for the IFDO assay (see text for details): Mycoplasma Broth, Tween 80, lysed blood, pancreatin 10 % (4× USP), horse serum, seeded with 0.1 µM of HLCs, incubated for 5–7 days. Results of 3–5 independent experiments
(s/h) replacement of Human blood with sheep (s) or horse (h) blood, Haem lysed blood substituted with haemin (5 mM) in DMSO, Pan 2.5 % pancreatin 10 % substituted with pancreatin 2.5 %

ISSN: 1475-2875