Fig. 2

ELISA for the determination of antibody titres in serum samples derived from control (un-immunized), WT-GVNP and Rec-GVNP-immunized mice. Plates were coated with (a) WT-GVNPs and (b) Rec-GVNPs. The error bars represent standard deviation for the triplicates. Control (un-immunized) sera showed no reactivity. Reactivity towards WT-GVNPs was similar in the two groups (p > 0.21) (WT-GVNP and Rec-GVNP-immunized) a; however, reactivity towards Rec-GVNPs was significantly higher (p < 0.002) b. Number of mice in each group were—Control n = 5, WT-GVNP n = 5; Rec-GVNP n = 10. Seven mice with highest anti-rPfeno antibody titres in the Rec-GVNP immunized group were selected for the parasite challenge