Fig. 1
From: Quantification of Plasmodium ex vivo drug susceptibility by flow cytometry
Gating strategy for mature schizonts. Red blood cells (RBCs) were identified and gated based on the forward/side scatter (FSC-H/SSC-H) dot plot in gate ‘RBCs’. The RBCs were visualized in a FSC-A/FSC-H dot plot to select cell singlets in gate ‘Single RBCs’. The single RBCs were then analysed in a SSC-H/Hydroethidine-H (FL2-H) dot plot for P. falciparum and a SSC-H/SYBR Green 1-H (FL1-H) dot plot for P. vivax. Mature schizonts were identified in gate ‘MS’ based on fluorescence intensity and side scatter values (i.e., increased DNA content and cell complexity) produced by schizonts compared to other Plasmodium life cycle stages