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Table 1 Plasmodium falciparum oocyst prevalence determined by microscopy (day 8 PI) and by the detection of CSP in the ECL-SB and colorimetric ELISA in separate mosquito samples processed 8 and 10 days PI from the same four experimental feeds

From: A comparison of Plasmodium falciparum circumsporozoite protein-based slot blot and ELISA immuno-assays for oocyst detection in mosquito homogenates

Group

Day PI

Microscopy

ECL-SB

ELISA

Mean oocysts (range)

Prevalence  %

95 % CI

n/N

Prevalence  %

95 % CI

n/N

p

Prevalence  %

95 % CI

n/N

p

A

8

0.5 (2–4)

20.8

7.1–42.2

5/24

27.8

14.2–45.2

10/36

0.54

0.0

0–9.7

0/36

0.00

10

–

–

–

–

25.0

12.1–42.2

9/36

0.71

33.3

18.6–51

12/36

0.29

B

8

0.9 (1–3)

40.0

19.1–63.9

8/20

37.5

24–52.6

18/48

0.85

0.0

0–7.4

0/48

0.00

10

–

–

–

–

39.6

25.8–54.7

19/48

0.97

43.8

29.5–58.8

21/48

0.78

C

8

6.1 (1–22)

70.0

50.6–85.3

21/30

75.0

57.8–87.9

27/36

0.65

11.1

3.1–26.1

4/36

0.00

10

–

–

–

–

80.6

64–91.8

29/36

0.32

75.0

57.8–87.9

27/36

0.65

D

8

7.7 (1–17)

93.3

77.9–99.2

28/30

91.7

80–97.7

44/48

0.79

14.6

6.1–27.8

7/48

0.00

10

–

–

–

–

87.5

74.8–95.3

42/48

0.41

87.5

74.8–95.3

42/48

0.41

  1. Group group of mosquitoes fed on the same gametocyte culture, Mean oocysts (range) mean oocysts is given as the mean of all mosquitoes sampled. Range is given as the range of oocyst numbers in positive infections, Day PI day post infection, n/N positive mosquitoes/total mosquito sample size, P Chi squared test p value for homogeneity between positivity rates in the ECL-SB and ELISA relative to microscopy