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Fig. 5 | Malaria Journal

Fig. 5

From: Beninese children with cerebral malaria do not develop humoral immunity against the IT4-VAR19-DC8 PfEMP1 variant linked to EPCR and brain endothelial binding

Fig. 5

Static inhibition assay of IEs on immobilized EPCR and human brain endothelial cells. a Adhesion inhibition assays on EPCR-coated plastic spots were performed using FCR3-VAR19 and FCR3-EPCR IEs pre-incubated with anti-VAR19 antibodies at different concentrations (ranging from 0.03 to120 µg/ml). The percentage of inhibition was calculated using the number of IEs obtained after pre-incubation with PBS alone as a reference. Error bars represent the standard deviation of three independent experiments. b Adhesion of FCR3-VAR19, FCR3-EPCR and FCR3-CSA IEs to HBEC5i cells in the presence or absence of anti-VAR19 antibodies is shown. Arrows points to adherent IEs. c Mean number of IEs adhering per 100 HBEC5i cells was determined in two independent assays (error bars represents the standard deviation). The percentage of inhibition was calculated using the number of IEs obtained after pre-incubation with PBS alone (no IgG) as a reference

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Malaria Journal

ISSN: 1475-2875

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