Fig. 1
From: Functional analysis of monoclonal antibodies against the Plasmodium falciparum PfEMP1-VarO adhesin
Surface reactivity of anti-PfEMP1-VarO mAbs assessed by flow cytometry. a Mean fluorescence intensity (MFI) of each anti-PfEMP1 VarO mAb reacting with the PfEMP1-VarO protein displayed on the surface of monovariant Palo Alto 89F5 VarO rosette-forming iRBC (trophozoite stage). Each mAb was used at 10 μg mL−1 and surface reactivity was monitored by indirect immunofluorescence using a goat anti-mouse IgG Alexa Fluor 488-conjugated antibody. The shaded histograms show labelling with a pool of mouse non-immune sera. b Titration curves of surface reactivity for individual mAbs, which are colour-coded as indicated