Fig. 6

In vitro binding assays showed a concentration dependent interaction between PfLisH and PfMDM2. Representative images of a isolated and purified recombinant GST-PfMDM2 and GST-PfSWIB, migrating at ~33 and ~42 kDa, respectively, and identified by Western blot analysis using an anti-GST antibody (marked with star); b stained SDS polyacrylamide gels and c binding curves showing the concentration-dependent association between a constant amount of immobilized PfLisH and increasing amounts of PfMDM2; and d stained SDS polyacrylamide gels and e binding curves showing the concentration-dependent association between a constant amount of immobilized PfARK3 and increasing amounts of PfSWIB