Fig. 3

Examples of differentially (alternatively) spliced introns between Plasmodium y. yoelii YM and P. y. nigeriensis NSM. cDNA reads from YM (Otto et al. [19]) and NSM were compared using IGV. Gene IDs are on top of each panel. Reads in pink are sense, and those in purple are antisense. a A coding intron present in NSM, but not in YM; b an intron in YM, but not in NSM; c, d 5′ UTR introns present in both YM and NSM, but the proportions of reads with spliced forms are different. For PYYM_0502300, most of the reads from NSM are spliced, but not those of YM (c). e additional sliced forms of 5′ UTR introns are present in YM, but not NSM; f an additional alternatively spliced coding intron in YM, but not NSM. YM may also have an extra 3′ UTR intron; g one intron in NSM, but multiple introns in antisense transcripts in YM, which may generate a large number of variants (>10)