Table 2 Primers and profiles used for amplification of pvdhps gene
Primer pairs (5′–3′) | Concentration of Mg2 (mM) | Concentration of primer (nM) | Annealing Temperature (°C/Min) | Cycle | Size (bp) |
|---|---|---|---|---|---|
VDHPS OF: ATTCCAGAGTATAAGCACAGCACATTTGAG VDHPS OR: CTAAGGTTGATGTATCCTTGTGAGCACATC | 2.0 | 250 | 69/2 | 21 | 1354 |
VDHPS NF: AATGGCAAGTGATGGGGCGAGCGTGATTGA VDHPS NR: CAGTCTGCACTCCCCGATGGCCGCGCCACC | 2.0 | 250 | 70.5/2 | 25 | 703 |
VDHPS 553OF: TTCTCTTTGATGTCGGCCTGGGGTTGGCCA VDHPS NR: CAGTCTGCACTCCCCGATGGCCGCGCCACC | 2.0 | 250 | 69.5/1 | 30 | 170 |