Fig. 5
From: An external sensing system in Plasmodium falciparum-infected erythrocytes
ItG pRBC were treated with TNF (1 ng ml−1), at the given a time points, followed by cell fractionation using combined methods of saponin lysis and detergent extraction. PfAB4 and other candidates’ expression and localization were revealed by Western blot. PfAB4 is localized in parasite cytoplasm, nuclear and insoluble fractions. In response to TNF treatment there was a transient shift from cytosol and insoluble compartments to the nucleus within 20–30 min. PGK and calcinurin also showed responsiveness to TNF treatment