Table 1 Primers for detection of human blood, bovine blood and malaria parasites
Species | Target gene | Primer name | Sequence (5′–3′) | PCR product size (bp) | References |
|---|---|---|---|---|---|
Homo sapiens (human) | rDNA | HUM1 | CGAGAGTTC//TCTGGAAGAATTGA | 519 | Mohanty et al. 2007 [16] |
HUM2 | TGATAGCCTGGAAGTGACAAAAT | ||||
Bovine Bos taurus | mtDNA | B1 | CATCATAGCAATTGCCATAGTCC | 165 | Corona et al. 2007 [20] |
B2 | GTACTAGTAGTATTAGAGCTAGAATTAG | ||||
Plasmodium (genus-wide) | 18S rRNA | rPLU5 | CCTGTTGTTGCCTTAAACTTC | 1100 | Johnston et al. 2006 [18] |
rPLU6 | TTAAAATTGTTGCAGTTAAAACG | ||||
P. falciparum (species specific) | 18S rRNA | rFAL1 | TTAAACTGGTTTGGGAAAACCAAATATATT | 205 | |
rFAL2 | ACACAATGAACTCAATCATGACTACCCGTC | ||||
P. vivax (species specific) | 18S rRNA | rVIV1 | CGCTTCTAGCTTAATCCACATAACTGATAC | 120 | |
rVIV2 | ACTTCCAAGCCGAAGCAAAGAAAGTCCTTA |