Table 1 Plasmodium spp. that could be amplified and hybridised by the primers and probe designed for POCKIT-iiPCR assay

P. falciparum

Humans

P. vivax

Humans

P. ovale

Humans

P. malariae

Humans, monkeys

P. knowlesi

Humans, monkeys

P. inui

Monkeys

P. fieldi

Monkeys

P. coatneyi

Monkeys, humans

P. cynomolgi

Monkeys, humans

P. fragile

Monkeys

P. simiovale

Monkeys

P. simium

Monkeys, humans

P. gonderi

Monkeys

P. hylobati

Monkeys

P. brasilianum

Monkeys

P. yoelii

Rodents

P. berghei

Rodents

P. chabaudi

Rodents

P. vinckei

Rodents

P. juxtanucleare

Birds

P. lophurae

Birds

Primer sequence

 Isothermo(F)

5′-CGGAAGGGCACCACCAG-3′

 Isothermo(R)

5′-TCACCATCCAAGAAATCAAGAAAG-3′

Amplicon size: 124 bp

Probe sequence

 Isoplasmo probe

FAM 5′-TCCTACTCTTGTCTTAAACTA-3′ MGB-NFQ