Evidence of new species for malaria vector Anopheles nuneztovari sensu lato in the Brazilian Amazon region

Malaria Journal

Table 4 Analysis of molecular variance (AMOVA) for testing the hierarchical population structure within Anopheles nuneztovari s.l. from the Brazilian Amazon region

COI					Microsatellites
Groups tested	Source of variation components	d.f.	Percentage variance (%)	Fixation index	Source of variation components	d.f.	Percentage variance (%)	Fixation index
No grouping (all) MN, CS, AU, TU, AP	Among samples	4	38.00	Φ _ST = 0.3800***	Among samples	1	8.08	F _ST = 0.081***
No grouping (all) MN, CS, AU, TU, AP	Within samples	98	62.00		Within samples	315	91.92
Two groups (1) MN, CS, AU (2) TU, AP	Between groups	1	30.93	Φ _CT = 0.3093	Between groups	1	6.03	F _CT = 0.060
	Among samples within groups	3	14.37	Φ _SC = 0.2081***	Among samples within groups	3	4.27	F _SC = 0.043***
	Within samples	98	54.70	Φ _ST = 0.4530***	Within samples	315	89.70	F _ST = 0.103***

See Table 1 for locality abbreviations
Significance test 10,000 permutations, d.f. degrees of freedom, Φ _ST fixation index within samples, Φ _CT fixation index between regions, Φ _SC fixation index among samples within regions, F _ST fixation index within samples, F _CT fixation index between regions, F _SC fixation index among samples within regions
*** P = 0.00000 ± 0.00000

ISSN: 1475-2875