Table 1 The primers and probe used for copy number quantification
From: Plasmodium copy number variation scan: gene copy numbers evaluation in haploid genomes
Name | Sequence | Gene amplification | Location |
|---|---|---|---|
CytbF | 5′GCACGCAACAGGTGCTTCTC 3′ | Cytochrome b | Mitochondira |
CytbR | 5′GACCCCATGGTAAGACATAACC 3′ | ||
CytBP | 5′(FAM)-CCATGATAATGGTAAATACATATATGAGTAATTT-(TAMRA) 3′ | ||
CLCPF | 5′GGGCCTAGTGGTACTGGTAA 3′ | clcp | Apicoplast |
CLCPR | 5′CCAACATAACCAGGAGGTGAACC 3′ | ||
CLCPP | 5′(FAM)-CATATCAAATCTAATTAGTTCTTTTTCAGAACC-(TAMRA) 3′ | ||
Mdr1F | 5′ TGCATCTATAAAACGATCAGACAAA 3′ | pfmdr1 | Nuclear |
Mdr1R | 5′ TCGTGTGTTCCATGTGACTGT 3′ | ||
Mdr1P | 5′ (FAM)-TTTAATAACCCTGATCGAAATGGAACCTTTG-(TAMRA) 3′ | ||
TubF | 5′AAAAATATGATGTGCGCAAGTGA 3′ | Pftubulin | |
TubR | 5′AACTTCCTTTGTGGACATTCTTCCT 3′ | ||
TubP | 5′ (TET)-TAGCACATGCCGTTAAATATCTTCCATGTCT-(TAMRA) 3′ |