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Fig. 6 | Malaria Journal

Fig. 6

From: Analysis of the dose-dependent stage-specific in vitro efficacy of a multi-stage malaria vaccine candidate cocktail

Fig. 6

Confirmation of the transmission-blocking activity of antibodies induced by VAMAX-Mix immunization by standard membrane feeding assay (SMFA). The SMFA was performed with total IgG purified from neutral rabbit serum (NRS, control, white), P1 (1 µg antigen dose, blue) and P50 (50 µg antigen dose, orange) at 1, 0.3, 0.1, 0.03 and 0.01 mg/ml, and the mean luminescence intensity values for 24 individual mosquitoes from a single experimental feeds are shown (the box indicate the 25th and 75th percentiles whereas the whiskers indicate minimum and maximum luciferase values). The transmission-blocking activity is expressed by comparing the mean luciferase activity of P1 and P50 with the corresponding luciferase activity in the NRS control group. The dose-dependency of the transmission-blocking activity was used to determine the IC50 values for total IgG as well as Pfs25-specific antibodies and the results are summarized in Table 3

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