Table 2 Differentially expressed genes in the parasite life cycle, and primers for qPCR
From: A novel validated assay to support the discovery of new anti-malarial gametocytocidal agents
Gene IDa | Description | Primersb |
|---|---|---|
Housekeeping gene | ||
18S rRNA | 18S ribosomal RNA (PF3D7_0112300) | AATAACAATGCAAGGCCAATTT CTGCAACAATTTTAATATACGC |
Young gametocytes | ||
PFD0310w | Sexual stage-specific protein precursor (Pfs16) | AGTTCTTCAGGTGCCTCTCTTCA AGCTAGCTGAGTTTCTAAAGGCA |
PF14_0748 | Exported protein (PHISTa), unknown function (Pfg14-748) | CTTATGTGCTGAATTTTGTGTTATGGT TGTAAACTCTGATTTGGCCACACT |
PF13_0011 | Gamete antigen 27/25 (Pf 27\25) | CATGAAACACATGCCCCTCTCT GCTACAGGCATGAAACTCAATATCC |
PF14_0744 | Exported protein, unknown function (Pfg14-744) | TTATGTATAATGGCTCTGTTGACGG GGCTTCTCGACTTCCTCGAA |
PFL0795c | Male development gene 1 (Pfmdv-1/peg3) | TTTGAGACATTTGAACAAGCTTTACA GCATTTCCGGATTTGTTATTTTC |
PF10_0164 | Early transcribed membrane protein 10.3 (ETRAMP10.3) | TGCTGCTGTTGCTTTGGCTA CCTCAGAGTCGGATCCATCATT |
Mature gametocytes | ||
PFF1035w | (Pf77) | GGAAGACAAAAAACACTGCACATTA TTCAAACCATCGTCCTCTTTTTC |
PFL2405c | Female specific gametocyte-specific (Pfg377) | TGTTCTTTTTCATATCGTCTATCTTCCT TTGCTTTCCTTAAGATGTTTAATGATG |
PF10_0303 | 25 kDa ookinete surface antigen precursor (Pfs25) | GACTGTAAATAAACCATGTGGAGA CATTTACCGTTACCACAAGTTA |
PFL2510w | Chitinase (pfcht1) | TCGAGCACGACCAGGTGAA CCTTTCCCACTCTCTTTAAATGTTTT |
PF10_0302 | 28 kDa ookinete surface protein (Pfs28) | AGAAAATGAAGTGTGTACATTAGAAGGAA GATGTATCAGCCTGGTCCACAGT |
MAL8.P1.16 | Rhomboid protease ROM3 (ROM3) | AAACTTGAGCACACCAAATGTTCAT CGGTAGCACAAGTCTCCAATATTG |