Table 1 Primers used in PCR
From: In vitro selection of Plasmodium falciparum Pfcrt and Pfmdr1 variants by artemisinin
Gene ID | Chromosome | Primers | Direction | PCR | size |
|---|---|---|---|---|---|
Pfcrt MAL7P1.27 | 5 | P1 (5′-gcgcgcgcatggctcacgtttaggtggag-3′) | Forward | Primary PCR | _146 bp |
P2 (5′-gggcccggcggatgttacaaaactatagttacc-3′) | Reverse | ||||
D1 (5′-tgtgctcatgtgtttaaactt-3′) | Forward | Nested PCR | |||
D2 (5′-caaaactatagttaccaattttg-3′) | Reverse | ||||
Pfmdr1 PFE1150w | 7 | Af (5′-tgtatgtgctgtattatcaggaggaac-3′) | Forward | Primary PCR | _560 bp |
Ar (5′-aattgtactaaacctatagatactaatgataatattatagg-3′) | Reverse | ||||
Bf (5′-gatggtaacctcagtatc-3′) | Forward | Nested PCR | |||
Br (5′-ctcctgataatacagcac-3′) | Reverse | ||||
Pfk13 PlasmoDB PF3D7_1218300 | 13 | K13-1 (5′-cggagtgaccaaatctggga-3′) | Forward | Primary PCR | 845 bp |
K13-4 (5′-gggaatctggtggtaacagc-3′) | Reverse | ||||
K13-2 (5′-gccaagctgccattcatttg -3′) | Forward | Nested PCR | |||
K13-3 (5′-gccttgttgaaagaagcaga -3′) | Reverse |