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Fig. 4 | Malaria Journal

Fig. 4

From: Functional genomic analyses of Enterobacter, Anopheles and Plasmodium reciprocal interactions that impact vector competence

Fig. 4

Differential gene expression in Esp_Z on different culture media and upon serial passage in the Anopheles gambiae midgut. a A parental strain of Esp_Z (P1) was provided to An. gambiae mosquitoes through a blood meal and sampled daily. Colonies persisting for the longest time were reintroduced into a second cohort (P2) and the process repeated a third time to isolate the passaged (P3) strain. The ability of a selected strain of Esp_Z to persist within the An. gambiae midgut was determined through qRT-PCR copy number analysis. b Distribution of the functional role category of upregulated (+) and downregulated (−) transcripts of parental P1 when compared to passaged P3. Functional role categories were assigned to 59/98 upregulated and 29/61 downregulated transcripts using the SEED database. c Distribution of the functional role category of upregulated (+) and downregulated (−) transcripts of Esp_Z grown in ookinete medium when compared to LB medium. Functional role categories were assigned to 15/21 upregulated and 14/20 downregulated transcripts using the SEED database

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