Fig. 4
Mice have IFN-γ-producing T cells in the brain during Plasmodium chabaudi infection. Compiled from two independent experiments, cells were isolated from PBS-perfused brains of female IL-10−/− (KO) and C57Bl/6J (WT) mice 7–9 days post-infection. a Representative FACS plots showing CD11b−CD45hi immune cells in the brain, gated on the Thy1.2+ population, then CD4 and CD8a. b Total cell numbers of CD4 and CD8 T cells within the CD11b−CD45hi/Thy1.2+/CD4+ or CD11b−CD45hi/Thy1.2+/CD8+ gate, respectively. c Histograms showing the expression of IFN-y in CD4+ and CD8+ T cells. Right panel MFI of IFN-y-expressing T cells in infected mice and uninfected controls. Percent positive proportion of infected IL-10−/− mice shown. Histogram colors Uninfected IL-10−/− (Grey); day 7 and day 9 post-infection WT (Blue); day 7–9 post-infection IL-10−/− (Red). I infected, U uninfected